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KMID : 0364819790170020058
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Korean Journal of Microbiology
1979 Volume.17 No. 2 p.58 ~ p.64
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Studies on some properties of cellulase isolated from pirieularia oryzae
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Abstract
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Studies on some properties of cellulase isolated from Piricularia oryzae. Crude cellulases were prepared from dried rice plant powder (Tong-il, Pal-dal) culture of P. oryzae(N-2, C-8, T-2). The best yield of enzyme was obtained from the medium using Tong-il rice plant powder for P. oryzae cav. N-2 and 2%-sucrose concentration in preculture media. Two units of the enzyme were incubated at 60℃ for 1hour with 1.0ml, 0.6% Na-CMC. The optimum temperature for the enzyme activity was at 60℃ and the optimum pH was at pH4.0. When Na-CMC₂was used as substrate the K_m values of crude enzyme were calculated to be 1.05 × 10^-4 mM and V_max was 2.8 mmole/hour. A 10-fold partial purification was achieved by (NH₄)₂SO₄precipitation followed by column chromatography on DEAE Sephadex A-25.
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